Amino acids of alfalfa mosaic virus coat protein that direct formation of unusually long virus particles

作者：Thole V; Miglino R; Bol JF^*

来源：Journal of General Virology, 1998, 79: 3139-3143.

In contrast to most alfalfa mosaic virus (AMV) strains (YSMV, S, M and 425), AMV strains VRU and 15/64 can form abnormally long virus particles, an ability which has been linked to the coat protein (CP). In order to study this phenomenon, the CP-encoding RNAs 3 of AMV strains VRU and 15/64 were cloned and fully sequenced. Comparative sequence analyses of AMV RNA 3 sequences derived from different strains revealed two non-conservative amino acid substitutions, Ser(66) and Leu(175), which occur exclusively in the closely related VRU- and 15/64-CPs. When these amino acid alterations were introduced into the CP of AMV strain 425 unusually long virus particles were assembled. This confirms that amino acids Ser(66) and Leu(175) of the CPs of AMV strains VRU and 15/64 are involved in the formation of tubular virus particles.

• 出版日期1998-12