科研之友
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摘要
Bacterial 6S RNA interacts specifically with RNA polymerase acting as transcriptional regulator. Until now, no detailed characterization of the spatial arrangement of the non-coding RNA within the three-dimensional structure of RNA polymerase has been performed. Here we present results obtained with the chemical nuclease FeBABE tethered to distinct positions of RNA polymerase sigma(70) subunit. 6S RNA complexes were formed with a collection of RNA polymerases, where the cleavage reagent had been fused to sigma(70) single-cysteine variants close to regions involved in promoter recognition. FeBABE-induced cleavage sites within the 6S RNA structure were identified, indicating close spatial neighborhood between sigma(70) single-cysteine side chains and defined positions of the 6S RNA structure. Our analysis demonstrates close proximity between the 6S RNA internal hairpin and sigma(70) domain 4.2, normally involved in recognition of -35 promoter DNA. Defined sections of the internal 6S RNA stem structure flanking the central bubble are positioned near conserved sigma(70) domains 3.1, 2.3 and 2.1, which are implicated in binding and melting DNA promoters between the -10 and -35 elements. Moreover, we show that U44 of 6S RNA is located near RNA polymerase active site (sigma(70) domain 3.2), fully consistent with its function as starting nucleotide in RNA-directed pRNA transcription. No neighboring contacts were detected between 6S RNA and sigma(70) region 1.2 or between sigma(70) and the 6S RNA closing stem structure (residues 1-41 and 144-184). Results were used to dock a structural model of 6S RNA to the known three-dimensional structure of Escherichia coli sigma(70) RNA polymerase holoenzyme.
- 出版日期2013-10-9
