Debranchase-resistant labeling of RNA using the 10DM24 deoxyribozyme and fluorescent modified nucleotides

作者:Carrocci Tucker J; Lohe Lea; Ashton Matthew J; Hoebartner Claudia; Hoskins Aaron A*
来源:Chemical Communications, 2017, 53(88): 11992-11995.
DOI:10.1039/c7cc06703h

摘要

The 10DM24 deoxyribozyme can site-specifically label RNAs with fluorophore-GTP conjugates; however, the 2 ',5 '-branched RNA linkage is readily cleaved by debranchase. To prevent loss of labels upon cleavage, we synthesized phosphorothioate-modified, fluorescent GTP derivatives and elaborated conditions for their incorporation by 10DM24. RNAs labeled with fluorescent derivatives of Sp-GTP(S) were found to be resistant to debranchase.

  • 出版日期2017-11-14