Amaryllidaceae Alkaloids Exhibit Anti-Influenza Activity in MDCK Cells, an Investigation of Amaryllidaceae Alkaloids and MDCK Cells Insight

作者：He, Jun; Qi, Wen-Bao; Tian, Jin; Jiao, Pei-Rong; Liu, Guo-Qian; Zhang, Chang-Hui; Liao, Ming^*

来源：Journal of Animal and Veterinary Advances, 2012, 11(14): 2485-2492.

• Amaryllidaceae alkaloid
• influenza A virus
• cytoskeleton
• apoptosis
• epidemics
• lycorine

Influenza A viruses occasionally cause large epidemics and kill thousands every year. While little is known about the mechanism of cell fusion in diseases, especially influenza virus infected and protection from Amaryllidaceae Alkaloids. The two compounds lycorine (AA1) and haemanthamine (AA3) were obtained from bulbs of L. radiate, exhibited anti-influenza activity after influenza virus entry cells. The two compounds were investigated for in vitro displaying different levels of resistance to pro-apoptotic stimuli. Seven influenza viruses were used, A/CK/GD/178/04 (H5N, 178), A/DK/GD/212/04 (H5N1, 212), A/swine/GD/166/06 (H3N2, 166), A/CK/HN/170/03 (H1N1, 170), A/Puerto Rico/8/34 (H1N1, PR8), A/Ck/GD/400/07 (H9N2, 400), A/Ck/GD/228/04 (H9N2, 228), the two compounds exhibited potency in the single-digit micromolar range. The studies also showed that AA1 and AA3 exerted their in vitro anti-influenza activity through cytostatic rather than cytotoxic effects. Many viruses interact with the host cells to change their own growth which they favor the speed. The cells infected with virus, growth of MDCK cells was slowed down by arresting cell cycle at G1/S phase. With the compound treated, the growth cycle was decreased in S phase. With H5N1 influenza virus treated, the cytoskeleton of cells was changed while with the compound treated the protection of cytoskeleton was obviously protected. The data showed differences between drug treated cells and virus infected cells, provided a basis to further explore cell fusion and anti-influenza mechanism of the two compounds.

• 出版日期2012
• 单位华南农业大学