Activation of the unfolded protein response in Pichia pastoris requires splicing of a HAC1 mRNA intron and retention of the C-terminal tail of Hac1p

作者：Whyteside Graham; Nor Rohana Mat; Alcocer Marcos J C; Archer David B^*

来源：FEBS Letters, 2011, 585(7): 1037-1041.

DOI：10.1016/j.febslet.2011.02.036

• Pichia
• Unfolded protein response
• UPR
• ER stress
• Protein secretion

We have shown that the unfolded protein response (UPR) in Pichia pastoris requires splicing of a non-conventional intron in the HAC1(u) mRNA in common with other eukaryotes. P. pastoris is a favoured yeast expression host for secreted production of heterologous proteins and the regulation of the UPR in P. pastoris may hold the key to its effective folding and secretion of proteins. We have also shown that the C-terminal region of the Hac1p from P. pastoris is required for functionality. Although the C-terminal regions of Hac1p from both S. cerevisiae and P. pastoris are rich in phenylalanine residues, the P. pastoris Hac1p lacks a C-terminal serine that is known to be important in the efficient functionality of Hac1p from S. cerevisiae.

• 出版日期2011-4-6