Analysis of PEG 400 in perfusate samples by aqueous normal phase (ANP) chromatography with evaporative light scattering detection

作者：Webster Gregory K^*; Elliott Ashley; Dahan Arik; Miller Jonathan M

来源：Analytical Methods, 2011, 3(3): 742-744.

DOI：10.1039/c0ay00698j

A rapid method for the analysis of polyethylene glycol (PEG 400) in perfusate samples is presented. Because PEG 400 lacks a strong chromophore in the UV range, detection was accomplished using evaporative light scattering detection (ELSD). In order to optimize the ELSD signal performance for a volatile mobile phase, the chromatographic separation was optimized using aqueous normal phase conditions on a Cogent (R) Diamond Hydride column.

• 出版日期2011-3