Comparative genome analysis between Southeast,Asian and South American Zika viruses

作者：Kochakarn Theerarat; Kotanan Namfon; Kum****sin Krittikorn; Loe**anluechai Duangkamon; Thammasatta Monta; Auewarakul Prasert; Wilairat Prapon; Chookajorn Thanat

来源：Asian Pacific Journal of Tropical Medicine, 2016, 9(11): 1026-1032.

DOI：10.1016/j.apjtm.2016.10.002

• Phylogenetic analysis
• Protein structure
• Southeast Asia
• Dengue virus
• Zika virus

Objective: To understand the cause for the differences between potentially mild Southeast Asian and the more pathogenic ZIKV in South America. Methods: A comparative genomic analysis was performed to determine putative causations stemming from ZIKV. Results: Phylogenctic analyses integrating geographical and time factors revealed that Southeast Asian ZIKV might not be the direct source of South American outbreaks as previously speculated. Amino acid residues unique to South American ZIKV isolates at the envelope, pr and NS1 proteins are listed and shown in the structural context. These unique residues on external viral proteins are not found in Southeast Asian ZIKV and could be responsible for the ongoing outbreak either via an intrinsic property of the virus or interactions with human immunity. Only a selected few primer/probe sets currently in clinical use were identified of being capable of detecting ZIKV strains worldwide. The envelope proteins of dengue virus (DENV) and ZIKV also showed a remarkable degree of similarity especially at the surface residues. Conclusions: findings that may help explain the cross-reactivity of DENY antibodies to ZIKV. Thus, major caveats must be exercised in using existing diagnostic tools for ZIKV.

• 出版日期2016-11