Acquisition of Thermotolerant Yeast Saccharomyces cerevisiae by Breeding via Stepwise Adaptation

作者：Satomura Atsushi; Katsuyama Yoshiaki; Miura Natsuko; Kuroda Kouichi; Tomio Ayako; Bamba Takeshi; Fukusaki Eiichiro; Ueda Mitsuyoshi^*

来源：Biotechnology Progress, 2013, 29(5): 1116-1123.

DOI：10.1002/btpr.1754

• thermotolerant yeast
• breeding
• trehalose
• Saccharomyces cerevisiae

A thermotolerant Saccharomyces cerevisiae yeast strain, YK60-1, was bred from a parental strain, MT8-1, via stepwise adaptation. YK60-1 grew at 40 degrees C, a temperature at which MT8-1 could not grow at all. YK60-1 exhibited faster growth than MT8-1 at 30 degrees C. To investigate the mechanisms how MT8-1 acquired thermotolerance, DNA microarray analysis was performed. The analysis revealed the induction of stress-responsive genes such as those encoding heat shock proteins and trehalose biosynthetic enzymes in YK60-1. Furthermore, nontargeting metabolome analysis showed that YK60-1 accumulated more trehalose, a metabolite that contributes to stress tolerance in yeast, than MT8-1. In conclusion, S. cerevisiae MT8-1 acquired thermotolerance by induction of specific stress-responsive genes and enhanced intracellular trehalose levels.

• 出版日期2013-9