A simple microfluidics for real-time plasma separation and hCG detection from whole blood

作者：Hung Min Sheng^*; Chang Hsiao Yun

来源：Journal of the Chinese Institute of Engineers, 2015, 38(6): 685-691.

DOI：10.1080/02533839.2015.1010455

• microfluidic device
• plasma separation
• whole blood
• hCG detection
• sheath flow

Plasma isolation and protein detection from whole blood are necessary processes for diagnosing various diseases and evaluating treatment effectiveness in patients. A microfluidics device has the potential to achieve the miniaturization and integration of blood detection assays. In this study, we have developed a simple microfluidics system for separating plasma and detecting human chorionic gonadotrophin (hCG) from whole blood using a sheath flow and polydimethylsiloxane as a material. We investigated the effects of various sheath flows on plasma separation and hCG detection. Our results showed that when the sheath flow increased, the number of blood cells in the plasma-recovered microchannels decreased. At a sheath flow 13.6x10(-5)m/s, the blood cells sedimented on the bottom of the main-flow microchannels. When the sheath flow was increased, the number of fluorescence spots indicating the detection of hCG decreased. Our results indicate that a prepared amount of protein A of 1x10(-2)mg/mL and a sheath flow of 13.6x10(-5)m/s provide the optimal operating conditions for successful plasma separation and increased fluorescence intensity of detected hCG.

• 出版日期2015-9