Sp1 mediates repression of the resistin gene by PPAR gamma agonists in 3T3-L1 adipocytes

作者：Chung S S; Choi H H; Cho Y M; Lee H K; Park K S^*

来源：Biochemical and Biophysical Research Communications, 2006, 348(1): 253-258.

DOI：10.1016/j.bbrc.2006.07.048

• resistin
• Sp1
• PPAR gamma
• thiazolidinediones
• O-glycosylation

Resistin is an adipokine related to obesity and insulin resistance. Expression of the resistin gene is repressed by the treatment of peroxisome proliferator-activated receptor gamma (PPAR gamma) agonists, thiazolidinediones (TZDs). In this study, we investigated the mechanism by which TZDs inhibit the resistin gene expression. Resistin gene expression was decreased by TZD in fully differentiated 3T3-L1 adipocytes, which was abolished after treatment of cycloheximide (a protein synthesis inhibitor). TZD could not repress the expression of the resistin gene in the presence of mithramycin A (an Sp1 binding inhibitor). Sp1 binding site of the resistin promoter (-122/ -114 bp) was necessary for the repression. Further investigation of the effect of TZDs on the modification of Sp1 showed that the level of O-glycosylation of Sp1 was decreased in this process. These results suggest that PPAR gamma activation represses the expression of the resistin gene by modulating Sp1 activity.

• 出版日期2006-9-15