Association between EBNA2 and LMP1 subtypes of Epstein-Barr virus and nasopharyngeal carcinoma in Thais

作者：Tiwawech Danai; Srivatanakul Petcharin; Karalak Anant; Ishida Takafumi^*

来源：Journal of Clinical Virology, 2008, 42(1): 1-6.

DOI：10.1016/j.jcv.2007.11.011

• nasopharyngeal carcinoma
• Epstein-Barr virus
• genotype
• del-LMP1
• Thailand

Background: Nasopharyngeal carcinoma (NPC) is an Epstein-Barr virus (EBV)-associated malignancy that is common in Thailand. The association of the EBV nuclear antigen 2 gene (EBNA2) and the latent membrane protein 1 gene (LMP1) with NPC is unclear.
Objective: To determine the association between EBNA2 and LMP1 subtypes of EBV and NPC in Thais.
Study design: EBV DNA from blood samples of 75 Thai patients with NPC and 44 age-matched controls was examined by PCR for EBNA2 subtypes (EBV type 1 and type 2) and LMP1 subtypes (wild-type LMP1 (wt-LMP1) and LMP1 containing a 30-bp deletion (del-LMP1)).
Results: The frequencies of EBV type 1 in cases (96.0%) and controls (97.7%) were similar. The frequency of del-LMP1 in cases (58.7%) was higher than in controls (36.4%) (p = 0.023). Among NPC patients, the frequency of del-LMP1 in stages III and IV (65.6%) was higher than in stages I and II (18.2%) (p = 0.006). By analyzing nucleotide sequence of the LMP1 region, a new subtype, Thai1, was identified.
Conclusion: LMP1 subtypes of EBV may be involved in NPC susceptibility. The del-LMP1 variant may be a useful molecular marker for early detection, diagnosis and prognosis of Thai patients with NPC.

• 出版日期2008-5