Detection and quantification of airborne inoculum of Blumeria graminis f. sp. tritici using quantitative PCR

作者：Cao, Xueren; Yao, Dongming; Zhou, Yilin^*; West, Jonathan S.; Xu, Xiangming; Luo, Yong; Ding, Kejian; Fan, Jieru; Duan, Xiayu

来源：European Journal of Plant Pathology, 2016, 146(1): 225-229.

DOI：10.1007/s10658-016-0908-8

• Blumeria graminis f. sp tritici
• Wheat powdery mildew
• Real-time PCR
• Airborne conidia
• Spore trap

In this study, a real-time PCR assay was established to quantify airborne inoculum of Blumeria graminis f. sp. tritici (Bgt), the causal agent of wheat powdery mildew. Burkard 7-day recording spore traps were used to collect Bgt spores in air in Langfang City, Hebei Province, China. A linear relationship between number of spores counted with a compound microscope and those determined with the real-time PCR assay was obtained in artificially inoculated spore trap tape sections. The dynamics of airborne conidia concentrations of Bgt were similar. There were significant correlations (P < 0.01) between spore concentrations by using a compound microscope and the real-time PCR assay in three growing seasons. The results demonstrated a potential method to quantitatively determine spore inoculum potential in wheat fields by using a real-time PCR assay.

• 出版日期2016-9
• 单位安徽农业大学; 植物病虫害生物学国家重点实验室; 中国热带农业科学院椰子研究所; 中国热带农业科学院; 中国农业大学; 中国农业科学院