A genetically encoded probe for cysteine sulfenic acid protein modification in vivo

作者：Takanishi Christina L; Ma Li Hua; Wood Matthew J^*

来源：Biochemistry, 2007, 46(50): 14725-14732.

DOI：10.1021/bi701625s

All organisms have defense mechanisms to combat the deleterious effects of oxidative damage produced by reactive oxidative species (ROS). Although it is known that ROS play a major role in oxidative damage, increasing evidence reveals that ROS have wider cellular effects through their role in many signal transduction pathways. Here we have adapted a redox-regulated domain from the Yap1 transcription factor in Saccharomyces cerevisiae to function as a general trap for proteins that form cysteine sulfenic acid (Cys-SOH) in vivo. In response to H2O2, the Yap1 probe forms mixed disulfide bonds with a variety of proteins. The formation of these protein complexes is time dependent and peroxide concentration dependent. Disulfide-bonded complex formation can be attenuated by the addition of dimedone, a compound that specifically reacts with Cys-SOH, indicating the specificity of the probe toward Cys-SOH. An efficient one-step purification procedure was developed for proteins trapped by the Yap I probe, and the constituents were identified by mass spectrometry. This methodology identified six proteins in Escherichia coli that contain redox-active cysteine residues known to form Cys-SOH as part of their catalytic cycle. The results suggest that the Yap1 probe is useful for identifying Cys-SOH-regulated proteins and can be employed in any genetically tractable organism to monitor transient Cys-SOH formation in vivo.

• 出版日期2007-12-18