Lung Fibroblasts Share Mesenchymal Stem Cell Features Which Are Altered in Chronic Obstructive Pulmonary Disease via the Overactivation of the Hedgehog Signaling Pathway

作者：Figeac Florence; Dagouassat Maylis; Mahrouf Yorgov Meriem; Le Gouvello Sabine; Trebeau Celine; Sayed Angeliqua; Stern Jean Baptiste; Validire Pierre; Dubois Rande Jean Luc; Boczkowski Jorge; Mus Veteau Isabelle; Rodriguez Anne Marie^*

来源：PLos One, 2015, 10(3): e0121579.

DOI：10.1371/journal.pone.0121579

Background
Alteration of functional regenerative properties of parenchymal lung fibroblasts is widely proposed as a pathogenic mechanism for chronic obstructive pulmonary disease (COPD). However, what these functions are and how they are impaired in COPD remain poorly understood. Apart from the role of fibroblasts in producing extracellular matrix, recent studies in organs different from the lung suggest that such cells might contribute to repair processes by acting like mesenchymal stem cells. In addition, several reports sustain that the Hedgehog pathway is altered in COPD patients thus aggravating the disease. Nevertheless, whether this pathway is dysregulated in COPD fibroblasts remains unknown.
Objectives and Methods
We investigated the stem cell features and the expression of Hedgehog components in human lung fibroblasts isolated from histologically-normal parenchymal tissue from 25 patients-8 non-smokers/ non-COPD, 8 smokers-non COPD and 9 smokers with COPD-who were undergoing surgery for lung tumor resection.
Results
We found that lung fibroblasts resemble mesenchymal stem cells in terms of cell surface marker expression, differentiation ability and immunosuppressive potential and that these properties were altered in lung fibroblasts from smokers and even more in COPD patients. Furthermore, we showed that some of these phenotypic changes can be explained by an over activation of the Hedgehog signaling in smoker and COPD fibroblasts.
Conclusions
Our study reveals that lung fibroblasts possess mesenchymal stem cell-features which are impaired in COPD via the contribution of an abnormal Hedgehog signaling. These processes should constitute a novel pathomechanism accounting for disease occurrence and progression.

• 出版日期2015-3-27