Deinococcus radioresistens sp nov., a UV and gamma radiation-resistant bacterium isolated from mountain soil

作者：Srinivasan Sathiyaraj; Lee Jae Jin; Lim Sang Yong; Joe Min Ho; Im Seong Hun; Kim Myung Kyum^*

来源：Antonie van Leeuwenhoek, 2015, 107(2): 539-545.

DOI：10.1007/s10482-014-0350-x

• Deinococcaceae
• Deinococcus radioresistens
• Radiation-resistant
• Taxonomy

Two Gram-negative, non-motile, short rod-shaped bacterial strains, designated as 8A(T) and 28A, were isolated from Mount Deogyusan, Jeonbuk Province, South Korea. The isolates were analyzed by a polyphasic approach, revealing variations in their phenotypic characters but high DNA-DNA hybridisation values reciprocally, confirming that they belong to the same species. Both the isolates also showed a high resistance to UV compared with Deinococcus radiodurans, and a gamma-radiation resistance similar to other members of the genus Deinococcus. Phylogenetic analysis with the 16S rRNA gene sequences of closely related species indicated their similarities were below 97 %. Chemotaxonomic data showed the most abundant fatty acids to be C-16:1 omega 7c and C-16:0. The strains can be distinguished from closely related species by the production of esterase (C4) and alpha-galactosidase, and by their ability to assimilate l-alanine, l-histidine and N-acetyl-d-glucosamine. Based on the phenotypic, phylogenetic, and chemotaxonomic data, the isolates represent a novel species of the genus Deinococcus, for which the name Deinococcus radioresistens sp. nov. is proposed. The type strain is 8A(T) (KEMB 9004-109(T) = JCM 19777(T)), and a second strain is 28A (KEMB 9004-113 = JCM 19778).

• 出版日期2015-2