Determination of the mobility of amine- and carboxy-terminated fluospheres and quantum dots by capillary electrophoresis

作者：Tekrony Amy; Cramb David^*

来源：Canadian Journal of Chemistry, 2016, 94(4): 430-435.

DOI：10.1139/cjc-2015-0349

• nanoparticles
• predictive pharmokinetics
• nanoparticle zeta potential
• capillary electrophoresis
• nanoparticle mobility
• polystyrene nanospheres
• quantum dots

The pharmacokinetics of nanoparticle (NP) theranostics can, in principle, be predicted based on NP size and zeta potential. Zeta potentials are typically measured using bench top zetasizer instruments, which calculate zeta potential based on mobility data collected from solutions in a small sample cell. However, correlations between zeta potentials measured by zetasizer instruments and those calculated from mobilities determined by instruments designed for capillary electrophoresis may not be direct. To that end, mobilities of a variety of NPs were determined by a capillary electrophoresis and used to calculate zeta potentials based on Henry's equation. The calculated zeta potentials were then compared to zeta potentials measured directly from a zetasizer. It was found that absolute values of the two methods differed, but the relative zeta potential trends per particle type were similar. These trends were demonstrated by data that showed that the zeta potentials measured using a zetasizer correlated highly with zeta potentials determined by capillary electrophoresis.

• 出版日期2016-4