Isolation and characterization of Micromonospora phage phi HAU8 and development into a phasmid

作者：Li, XH; Zhou, XF; Deng, ZX^*

来源：Applied and Environmental Microbiology, 2004, 70(7): 3893-3897.

DOI：10.1128/AEM.70.7.3893-3897.2004

• Lysogen
• Plasmids

PhiHAU8, a temperate Micromonospora phage, which is capable of infecting Micromonospora sp. strains 40027 and A-M-01, was isolated. The PhiHAU8 virion has a polyhedral head and a flexible tail and has a small genome (ca. 42.5 kb) with double-stranded DNA and cohesive ends. PhiHAU8 was most stable at 4degreesC in Difco nutrient broth within a pH range of 6 to 12. PhiHAU8 plaque formation on Micromonospora sp. strain 40027 was optimal with 32 mM Ca2 and 30 mM Mg2 . A lysogen, LXH8, was isolated from turbid plaques, and a phasmid derivative that functions as a lambda cosmid vector in Escherichia coli and as a phage in Micromonospora sp. strain 40027 was constructed. Pulsed-field gel electrophoresis of AseI-digested total DNA showed that PhiHAU8 DNA integrates into the 500-kb AseI fragment of Micromonospora sp. strain 40027.

• 出版日期2004-7
• 单位上海交通大学; 华中农业大学