Measuring Screw-Sense Preference in a Helical Oligomer by Comparison of C-13 NMR Signal Separation at Slow and Fast Exchange

作者：Sola Jordi; Morris Gareth A; Clayden Jonathan^*

来源：Journal of the American Chemical Society, 2011, 133(11): 3712-3715.

DOI：10.1021/ja1097034

While an unequal population of rapidly inter-converting left- and right-handed conformers of a helical oligomer can be detected by circular dichroism, precise quantification of a conformer ratio has not previously been achieved. We demonstrate, using a set of labeled peptide analogues, that simple analysis of peak separation in their C-13 NMR spectra at slow and fast exchange allows an accurate value for the ratio of helical conformers to be obtained. The method reports the ratio of conformers at the site of the label and can therefore be used to investigate local variations in helical conformational control.

• 出版日期2011-3-23