A neutral polyacrylate copolymer coating for surface modification of thiol-ene microchannels for improved performance of protein separation by microchip electrophoresis

作者：Me**ah Kiarach; Thanh Duc Mai; Jensen Thomas Glasdam; Sola Laura; Chiari Marcella; Kutter Jorg P; Taverna Myriam^*

来源：Microchimica Acta, 2016, 183(7): 2111-2121.

DOI：10.1007/s00604-016-1825-4

• Thiol-ene polymers
• Electro-osmotic flow
• Protein separation
• N, N-Dimethylacrylamide
• 3-(Methacryloyl-oxy) propyl-trimethoxysilane
• 3-Trimethylsilanyl-prop-2-yne methacrylate
• Surface hydrophilicity
• Protein adsorption

We have investigated the behavior of thiol-ene substrates that is a class of promising materials for lab-on-a-chip electrophoresis applications. Two polymeric materials were prepared by copolymerization of N, N-dimethylacrylamide (DMA), (3-(methacryloyl-oxy)propyl)trimethoxysilane (PMA) and 3-trimethylsilanyl-prop-2-yne methacrylate (MAPS) and specifically adapted to thiol-ene formulations in order to obtain a neutral and permanent coating for microchannels. The performance of two different thiol-ene substrates (with 20% and 40% excess of thiol groups, respectively) coated with either p-(DMA-PMA) or p-(DMA-PMAMAPS) copolymer were evaluated in terms of surface hydrophilicity, suppression and stability of electro-osmotic flow and prevention of protein adsorption. Surface modification of thiol-ene containing a 20 % excess of thiols with the terpolymer p-(DMA-PMA-MAPS) was found to offer the most stable coating and most efficient charge shielding in the pH range from 3 to 9. The modified microchannels were successfully applied to electrokinetic separations of acidic and basic proteins.

• 出版日期2016-7