A kind of integrated microfluidic system for rapid pathogenic Botrytis cinerea detection

作者：Xu, P. F.; Yang, N.^*; Jia, J.; Xu, C.; Guo, J. J.

来源：Bulgarian Chemical Communications, 2017, 49(1): 264-268.

• Pathogens
• formatting
• Integrated microfluidic
• Rapid detection
• Impedance

In order to overcome the poor timeliness and low automaticity of the detection of existing Botrytis cinerea in fruits and vegetables, an integrated microfluidic system for rapid detection is proposed. In this system, the microelectrode is mounted on the bottom of the microfluidic chip channel and connected to a digital impedance measurement circuit. The magnetic beads whose surface is encased by mouse Botrytis cinerea antibody, is controlled by an external Gauss magnetic field to capture Botrytis cinerea. Then the combination of Botrytis cinerea and the magnetic beads is transferred to a microelectrode array. By constructing a counting circuit based on impedance measurement at the electrode terminals, the amount of Botrytis cinerea can be effectively measured. The results show the validity of the system in detecting the amount of Botrytis cinerea in fruits and vegetables. In addition, the detection time is only nearly one- fiftieth compared with a traditional laboratory test.

• 出版日期2017
• 单位江苏大学; 江苏农林职业技术学院