New effects and applications of thioflavins

作者：Lakatos Boris^*; Kalinakova Barbora; Hudecova Daniela; Varecka Ludovit

来源：Central European Journal of Biology, 2010, 5(2): 143-150.

DOI：10.2478/s11535-010-0008-2

• Thioflavin T
• Thioflavin S
• Trichoderma viride
• Growth
• Conidiation

The thiazol dye Thioflavin T (ThT), which is used to stain amyloid fibrils, was found to have strong inhibitory effects on both growth and conidiation of the deuteromycete Trichoderma viride at concentrations between 10-100 A mu g/ml (ca. 30-300 A mu mol/l). Thioflavin S (ThS), also known to stain amyloid fibrils, had no significant effect at these concentrations. Both stains yielded a fluorescence response, but their distributions were different. ThT was non-homogenously distributed throughout the cytoplasm, whereas ThS fluorescence was strongly bound to septal regions. The effect of ThT was studied on several model microorganisms. It exerted a strong inhibitory effect on Staphylococcus aureus (Gram-positive bacterium) (MIC=10 A mu mol/l), but the effect on Escherichia coli (Gram-negative bacterium) was one order of magnitude less pronounced. The effect on Candida albicans was also very strong (MIC=50 A mu mol/l). The dermatophytic fungus Microsporum gypseum and deuteromycete Alternaria alternata were less affected by ThT (MIC=250 A mu mol/l and > 500 A mu mol/l, respectively). These results show that ThT could be a useful inhibitor of selected microorganisms, whereas ThS could be a useful agent for monitoring formation and maintenance of intrahyphal septa without inhibiting the growth of the microorganism.

• 出版日期2010-4