Fast, Temperature-Sensitive and Clathrin-Independent Endocytosis at Central Synapses

作者：Delvendahl Igor^*; Vyleta Nicholas P; von Gersdorff Henrique; Hallermann Stefan^*

来源：Neuron, 2016, 90(3): 492-498.

DOI：10.1016/j.neuron.2016.03.013

The fusion of neurotransmitter-filled vesicles during synaptic transmission is balanced by endocytotic membrane retrieval. Despite extensive research, the speed and mechanisms of synaptic vesicle endocytosis have remained controversial. Here, we establish low-noise time-resolved membrane capacitance measurements that allow monitoring changes in surface membrane area elicited by single action potentials and stronger stimuli with high-temporal resolution at physiological temperature in individual bona-fide mature central synapses. We show that single action potentials trigger very rapid endocytosis, retrieving presynaptic membrane with a time constant of 470 ms. This fast endocytosis is independent of clathrin but mediated by dynamin and actin. In contrast, stronger stimuli evoke a slower mode of endocytosis that is clathrin, dynamin, and actin dependent. Furthermore, the speed of endocytosis is highly temperature dependent with a Q(10) of similar to 3.5. These results demonstrate that distinct molecular modes of endocytosis with markedly different kinetics operate at central synapses.

• 出版日期2016-5-4