Characterization of Listeria monocytogenes Isolates from Foods by Molecular Subtyping

作者：Wang, Wenkai; Chen, Wanyi; Suo, Yujuan; Zhou, Xiujuan; Shi, Chunlei; Shi, Xianming^*

来源：Journal of Chinese Institute of Food Science and Technology, 2017, 17(2): 220-226.

DOI：10.16429/j.1009-7848.2017.02.029

• ERIC-PCR
• Food isolates
• Food-borne pathogens
• Listeria monocytogenes
• Monocytogenes
• Reference strains
• Serotypes
• Similarity coefficients

Listeria monocytogenes is a major foodborne pathogen, which generally causes human illness including meningitis, meningoencephalitis and abortion. In this study, our aim was to characterize a total of 86 L. monocytogenes food isolates in Shanghai by serotyping and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) subtyping analyses. All the isolates were serotyped by multiplex PCR assays into three serogroups: 1/2a or 3a (72.1%, n=62), 1/2b or 3b or 7 (19.8%, n=17) and 1/2c or 3c (8.1%, n=1). At the relative similarity coefficient of 0.80, 86 isolates and 4 reference strains were identified into 10 clusters and 8 singletons by ERIC-PCR. It was found that the L. monocytogenes isolates sharing the same serogroup could be clustered together by ERIC-PCR at a certain relative similarity coefficient, while isolates belonging to the same serogroup could be efficiently distinguished according to diverse ERIC-PCR profiles. Furthermore, our results will provide the fundamental data for epidemiological surveillance of L. monocytogenes.

• 出版日期2017
• 单位上海交通大学