Molecular cloning and expression of a 2-Cys peroxiredoxin gene in the crustacean Eurypanopeus depressus induced by acute hypo-osmotic stress

作者：Van Horn Julie; Malhoe Vedanta; Delvina Marlin; Thies Megan; Tolley S Gregory; Ueda Takashi^*

来源：Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology, 2010, 155(3): 309-315.

DOI：10.1016/j.cbpb.2009.11.015

• Peroxiredoxin
• Crustaceans
• Osmoregulation
• Salinity
• Flatback mud crab

Peroxiredoxins (Prxs) are a family of ubiquitous proteins that help minimize the harmful effects of oxidative stress by catalyzing the reduction of hydrogen peroxide (H(2)O(2)) and organic hydroperoxides to less harmful forms. A full-length cDNA corresponding to a 2-Cys Prx gene was isolated from the flatback mud crab Eurypanopeus depressus and designated as EdPrx-1 (GenBank accession no. EU684547). EdPrx-1 has a major open-reading frame of 594 bp and is capable of encoding a polypeptide of 198 amino acid residues. Like other 2-Cys Prxs, EdPrx-1 protein possesses two conserved cysteine residues that play an essential role for the antioxidant activity of the proteins. The EdPrx-1 protein, as deduced from the cDNA sequence, shows a high level (74-93%) of sequence similarity to the 2-Cys Prxs from other crustaceans as well as those from many arthropod species (73-76% similarity). It shares about 70% sequence similarity with homologs from mammalian species. EdPrx-1 gene is expressed at low level in the gill, hypodermis, and hepatopancreas tissues of the crab under non-stressed condition; however, its expression is elevated about three-fold in the gills under hypo-osmotic stress. This suggests a possible role in protecting against oxidative stress caused by the increased metabolic activities associated with hyperosmoregulation.

• 出版日期2010-3