Multilineage differentiation of muscle-derived stem cells from GFP transgenic mice

作者：Ye Chuan^*; Li Jiangwei; He Zixu; Nin Xu; Zhang Yi; Shang Xianwen; Liu Riguang; Duan Yiqiang

来源：Biotechnology Letters, 2010, 32(11): 1745-1752.

DOI：10.1007/s10529-010-0344-3

• Chondrogenic
• Green fluorescent protein
• Muscle-derived stem cells
• Myogenic cells
• Neurogenic cells

It is unclear whether green fluorescent protein (GFP) expression is maintained during the course of multilineage differentiation of muscle-derived stem cells (MDSCs). We isolated MDSCs from GFP-transgenic mice and transferred them to chondrogenic, neurogenic or myogenic media. Multilineage differentiation was examined by morphological observation, histological staining, immunocytochemical staining, real-time RT-PCR and Western blot. Both differentiated cells and non-differentiated cells maintained stable GFP expression until the cells exhibited a senescent phenotype. Thus, MDSCs from GFP-transgenic mice have multilineage potential in vitro and that GFP expression does not influence the multilineage potential of MDSCs (or vice versa).

• 出版日期2010-11
• 单位贵州医科大学