科研之友
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摘要
For research on inhomogeneous cells, we present a simulation method called the dual-medium quantitative (DMQ) measurement simulation method, which is realized by combining phase-shifting digital holography with DMQ analysis. The reliability of this method is confirmed by comparing the simulated phase map with the experimental one by the Hilbert phase microscope [J. Phys. Chem. A 113, 13327 (2009)], and its ability for studying inhomogeneous cells is demonstrated with measurements of a simulated HeLa cell. The average deviation and the relative deviation of physical thickness and axially averaged refractive index are 0: 0339 mu m, 0.69% and 0.0013, 0.094%, respectively. This approach can provide good guidance for experimental research on inhomogeneous cells.
- 出版日期2011-12-10
- 单位江苏大学
