科研之友
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摘要
Fusarium ear rot is a destructive disease in maize mostly caused by the fungus Fusarium verticillioides (FV), which results in reduction of grain yield. To investigate the relationship between the responsive genes during FV infection and QTL chromosome regions for resistance to ear rot in maize, the differentially expressed genes and resistant QTL were co-localized on the reference map IBM2 neighbors 2008 (Intermated B73xMo17 Map) in the maize chromosome regions. One hand, gene expression changes was investigated through suppression subtractive hybridization (SSH) and Genechip experiments in resistant inbred line Bt-1, which derived from the tropical germplasm with high resistance to Fussrium ear rot in southwest China. On the other hand, 117 initial QTLs associating with resistance to Fusarium ear rot from early documents were collected to project meta-QTL on the high-density genetic linkage map IBM2. In total, 312 single-copy FV-induced sequences were obtained from the above two methods in Bt-1 and 29 meta-QTLs for ear rot resistance were projecteded in IBM2 map for distributing on chromosomes 1 to 8. Finally, the co-localization analysis results showed that 35 FV-induced sequences were located in the meta-QTL regions, while most other sequences were closed with the meta-QTLs. This phenomenon indicates that the correlation between FV-induced genes and ear rot resistance was connected together closely by their comparing localization. The co-localization analysis also indicated that FV-induced sequences were clustered on the same chromosome regions, particularly on bins 3.06, 3.04 and 6.02. These regions of interest on chromosomes were recommended to use for improving ear rot resistance in a marker assisted selection (MAS) program.
- 出版日期2013
- 单位四川农业大学
