A colorimetric and fluorogenic probe for bisulfite using benzopyrylium as the recognition unit

作者：Zhang, Yun; Zhang, Xiangwen; Yang, Xiao-Feng^*; Zhang, Juan^*

来源：Luminescence, 2017, 32(7): 1233-1239.

DOI：10.1002/bio.3316

• benzopyrylium
• bisulfite
• coumarin
• fluorescent probe

A coumarin-benzopyrylium (CB) platform has been developed for the colorimetric and fluorogenic detection of bisulfite. The proposed probe utilizes coumarin as the fluorophore and positively charged benzopyrylium as the reaction site. The method employs the nucleophilic addition of bisulfite to the benzopyrylium moiety of CB to inactivate the electron-deficient oxonium ion. The driving force for photo-induced electron transfer is considerably diminished, thereby promoting the emission intensity of the coumarin fluorophore. The fluorescence intensity at 510nm is linear with bisulfite concentration over a range of 0.2-7.5M with a detection limit of 42nM (3). CB shows a rapid response (within 30s) and high selectivity and sensitivity for bisulfite. Preliminary studies show that CB has great potential for bisulfite detection in real samples and in living cells.

• 出版日期2017-11
• 单位西北大学