Disruption of sphingolipid biosynthetic gene IPT1 reduces Candida albicans adhesion and prevents activation of human gingival epithelial cell innate immune defense

作者：Rouabhia Mahmoud^*; Mukherjee Pranab K; Lattif Ali Abdul; Curt Severine; Chandra Jyotsna; Ghannoum Mahmoud A

来源：Medical Mycology, 2011, 49(5): 458-466.

DOI：10.3109/13693786.2010.535031

• IPT1 gene
• Sphingolipid
• Candida
• epithelial cells
• oral mucosa
• TLRs
• defensins

We demonstrated the effect of a Candida albicans sphingolipid biosynthetic gene, IPT1, on the interaction between gingival epithelial and Candida cells using monolayer cultures and engineered human oral mucosa tissue (EHOM). Disrupting the IPT1 gene greatly reduced Candida adhesion to gingival epithelial cells, compared to the wild-type and revertant strains. The yeasts adhesion to epithelial cells may activate toll-like receptors (TLRs). Cell response against Candida infection was thus investigated by evaluating TLR expression and antimicrobial peptide (AMP) production. The wild-type and revertant strains both activated TLR2, TLR4, TLR6, and TLR9 gene expression in the epithelial cells, whereas the delta Delta ipt1 mutant Candida strain had no effect on this expression. This finding was supported by an increased AMP expression (human beta beta-defensin HBD-2 and HBD-3) in the EHOM tissue infected with the wild-type and revertant Candida strains, and a decreased expression in the delta Delta ipt1 mutant-infected model. HBD protein secretion confirmed the absence of any effect by the delta Delta ipt1 on epithelial cell innate defense. This is the first study to demonstrate that a disruption of the IPT1 gene affects Candida-host interaction, thus preventing TLR activation and beta beta-defensin expression.</.

• 出版日期2011-7