Nano-cerium-element-doped titanium dioxide induces apoptosis of Bel 7402 human hepatoma cells in the presence of visible light

作者：Wang, Long; Mao, Jian^*; Zhang, Gao-Hua; Tu, Ming-Jing

来源：World Journal of Gastroenterology, 2007, 13(29): 4011-4014.

DOI：10.3748/wjg.v13.i29.4011

• nano titanium dioxide
• cerium
• photocatalysis
• apoptosis
• hepatoma cells

AIM: To investigate the apoptotic effect of photoexcited titanium dioxide (TiO2) nanoparticles in the presence of visible light on human hepatoma cell line (Bel 7402) and to study the underlying mechanism.
METHODS: Cerium-element-cloped titanium dioxide nanoparticles were prepared by impregnation method. Bel 7402 human hepatoma cells were cultured in RPMI 1640 medium in a humidified incubator with 50 mL/L CO2 at 37 degrees C. A 15 W fluorescent lamp with continuous wavelength light was used as light source in the photocatalytic test. Fluorescence morphology and agarose gel eletrophoresis pattern were performed to analyze apoptotic cells.
RESULTS: The Ce (IV)-doped TiO2 nanoparticles displayed their superiority. The adsorption edge shifted to the 400-450 nm region. With visible light illuminated for 10 min, 10 mu g/cm(3) Ce (IV)-doped TiO2 induced micronuclei and significant apoptosis in 4 and, 24 h, respectively. Hochest 33258 staining of the fixed cells revealed typical apoptotic structures (apoptotic bodies), agarose gel electrophoresis showed typical DNA ladder pattern in treated cells but not in untreated ones.

• 出版日期2007-8-7
• 单位四川大学