Stem cells of the lamina propria of human oral mucosa and gingiva develop into mineralized tissues in vivo

作者：Treves Manusevitz Sandra; Hoz Lia; Rachima Heled; Montoya Gonzalo; Tzur Ephraim; Vardimon Alexander; Narayanan A Sampath; Amar Salomon; Arzate Higinio; Pitaru Sandu^*

来源：Journal of Clinical Periodontology, 2013, 40(1): 73-81.

DOI：10.1111/jcpe.12016

• bone
• cementum
• gingiva
• oral mucosa
• regeneration
• stem cells

Aims To characterize the mineralized tissue formed constitutively in the supracalvarial region of scid mice by a primitive stem cell population (hOMSC) derived from the lamina propria of the human oral mucosa and gingiva. %26lt;br%26gt;Material and Methods FibrinhOMSC constructs were cultured for 14 days at which time point they were analysed for the expression of osteoblastic/cementoblastic markers and implanted between the skin and calvaria bones into scid mice. After 8 weeks, the animals were sacrificed and the implantation sites analysed. %26lt;br%26gt;Results Two-week-old cultures of fibrinhOMSC constructs expressed osteogenic/cementogenic markers at the gene level. Macroscopic and radiographic examinations revealed mineralized masses at the implantation sites of fibrinhOMSC constructs. Histology, histochemistry and immunofluorescence showed mineralized masses consisting of avascular cellular and acellular matrices that stained positively for collagen, Ca, cementum attachment protein, cementum protein 1, bone sialoprotein, alkaline phosphatase, osteocalcin, amelogenin and ameloblastin. Positive anti-human nuclear antigen indicated the human origin of the cells. Atomic force microscopy depicted long prismatic structures organized in lamellar aggregates. %26lt;br%26gt;Conclusions Within the limitation of this study, the results indicate for the first time that fibrinhOMSC constructs are endowed with the constitutive capacity to develop into mineralized tissues that exhibit certain similarities to cementum and bone.

• 出版日期2013-1