A novel circular RNA, hsa_circ_0046701, promotes carcinogenesis by increasing the expression of miR-142-3p target ITGB8 in glioma

作者：Li, Guobin; Yang, Haiying; Han, Kun^*; Zhu, Dezhang; Lun, Peng; Zhao, Yan

来源：Biochemical and Biophysical Research Communications, 2018, 498(1): 254-261.

DOI：10.1016/j.bbrc.2018.01.076

• Glioma
• hsa_circ_0046701
• miR-142-3p
• ITGB8
• Proliferation
• Invasion

In the present study, we identified a novel circular RNA (circRNA), hsa_circ_0046701, in glioma cells. We measured the expression of hsa_circ_0046701 using qRT-PCR in glioma tissues and cell lines, and explored its functions using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, colony formation, and Transwell assays. Luciferase reporter assays were performed to validate the correlation between microRNA miR-142-3p and hsa_circ_0046701 or integrin subunit beta 8 (ITGB8). The results showed that hsa_circ_0046701 was significantly upregulated in glioma tissues and cell lines, and knockdown of hsa_circ_0046701 inhibited cell proliferation and invasion. Luciferase reporter assays indicated that hsa_circ_0046701 functions as a sponge for miR-142-3p and regulates the expression of ITGB8. Subsequently, functional assays revealed that silencing of hsa_circ_0046701 could upregulate miR-142-3p, resulting in downregulation of ITGB8. The results demonstrated that the hsa_circ_0046701 miR-142-3p/ITGB8 axis might play critical regulatory roles in the pathogenesis and development of glioma.

• 出版日期2018-3-25
• 单位青岛大学