A plant-transformation-competent BIBAC/BAC-based map of rice for functional analysis and genetic engineering of its genomic sequence

作者：Li Yaning; Uhm Taesik; Ren Chengwei; Wu Chengcang; Santos Teofila S; Lee Mi Kyung; Yan Bo; Santos Felipe; Zhang Aimin; Scheuring Chantel; Sanchez Alma; Millena Anna C; Nguyen Henry T; Kou Hongda; Liu Daqun; Zhang Hong Bin^*

来源：Genome, 2007, 50(3): 278-288.

DOI：10.1139/G07-006

• BIBAC
• functional genomics
• genetic engineering
• genetic transformation
• physical mapping
• positional cloning

Sequencing of the rice genome has provided a platform for functional genomics research of rice and other cereal species. However, multiple approaches are needed to determine the functions of its genes and sequences and to use the genome sequencing results for genetic improvement of cereal crops. Here, we report a plant-transformation-competent, binary bacterial artificial chromosome (BIBAC) and bacterial artificial chromosome (BAC) based map of rice to facilitate these studies. The map was constructed from 20 835 BIBAC and BAC clones, and consisted of 579 overlapping BIBAC/ BAC contigs. To facilitate functional analysis of chromosome 8 genomic sequence and cloning of the genes and QTLs mapped to the chromosome, we anchored the chromosomal contigs to the existing rice genetic maps. The chromosomal map consists of I I contigs, 59 genetic markers, and 36 sequence tagged sites, spanning a total of ca. 38 Mb in physical length. Comparative analysis between the genetic and physical maps of chromosome 8 showed that there are 3 "hot" and 2 "cold" spots of genetic recombination along the chromosomal arms in addition to the "cold spot" in the centromeric region, suggesting that the sequence component contents of a chromosome may affect its local genetic recombination frequencies. Because of its plant transformability, the BIBAC/BAC map could provide a platform for functional analysis of the rice genome sequence and effective use of the sequencing results for gene and QTL cloning and molecular breeding.

• 出版日期2007-3
• 单位中国科学院遗传与发育生物学研究所; 河北农业大学