Low-Frequency Microsatellite Instability in Genomic Di-Nucleotide Sequences Correlates with Lymphatic Invasion and Poor Prognosis in Gastric Cancer

作者：Zhao, Yan; Zheng, Zhi-Chao^*; Luo, Ya-Hong; Piao, Hao-Zhe; Zheng, Guo-Liang; Shi, Jing-Yi; Zhang, Tao; Zhang, Jian-Jun

来源：Cell Biochemistry and Biophysics, 2015, 71(1): 235-241.

DOI：10.1007/s12013-014-0189-9

• Microsatellite instability
• Stomach neoplasms
• Di-nucleotide
• Survival analysis

The clinical significance of low-frequency microsatellite instability (MSI-L) in gastric cancer (GC) has not been well established. The aim of this study was to evaluate the clinicopathological features of MSI-L in GC. We investigated microsatellite instability (MSI) in 5 di-nucleotide repeat sequences in 210 unselected GC patients. High-resolution fluorescent microsatellite analysis assay was utilized to detect MSI. Clinicopathological variables were compared among groups with different microsatellite statuses. The overall survival (OS) was analyzed by Kaplan-Meier method. Multivariable analysis was performed to identify prognostic factors and variables correlated with lymph node metastasis. High-frequency microsatellite instability (MSI-H), MSI-L, and microsatellite stable were identified, respectively, in 10.5, 10.0, and 79.5 % of unselected GC cases. Tumors with MSI-H were less invasive, and these patients showed a better OS. MSI-L was correlated with more advanced tumor Node Metastasis stage, and more frequent lymph node metastasis. The unfavorable prognosis predicted by MSI-L was ascribed to its correlation with lymphatic invasion. MSI-L characterized by di-nucleotide markers represents a distinct subcategory of GC with aggressive clinicopathological features, which are particularly affiliated to lymphatic system and correlated with a poor prognosis. MSI-L could be beneficial for predicting the clinical outcome of GC.

• 出版日期2015-1
• 单位辽宁省肿瘤医院; 大连医科大学