Approaches to deorphanization of human and microbial cytochrome P450 enzymes

作者：Guengerich F Peter^*; Tang Zhongmei; Cheng Qian; Salamanca Pinzon S Giovanna

来源：Biochimica et Biophysica Acta-Proteins and Proteomics, 2011, 1814(1): 139-145.

DOI：10.1016/j.bbapap.2010.05.005

• Cytochrome P450
• Enzyme function
• Mass spectrometry
• Metabolomics
• Secondary pathways

One of the general problems in biology today is that we are characterizing genomic sequences much faster than identifying the functions of the gene products, and the same problem exists with cytochromes P450 (P450). One fourth of the human P450s are not well-characterized and therefore considered "orphans." A number of approaches to deorphanization are discussed generally. Several liquid chromatography-mass spectrometry approaches have been applied to some of the human and Streptomyces coelicolor P450s. One current limitation is that too many fatty acid oxidations have been identified and we are probably missing more relevant substrates, possibly due to limits of sensitivity.

• 出版日期2011-1