科研之友
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摘要
In neurons, translational regulation of gene expression has been implicated in the activity-dependent management of synapto-dendritic protein repertoires. However, the fundamentals of stimulus-modulated translational control in neurons remain poorly understood. Here we describe a mechanism in which regulatory brain cytoplasmic (BC) RNAs cooperate with eukaryotic initiation factor 4B (elF4B) to control translation in a manner that is responsive to neuronal activity. elF4B is required for the translation of mRNAs with structured 5%26apos; untranslated regions (UTRs), exemplified here by neuronal protein kinase M zeta (PKM zeta) mRNA. Upon neuronal stimulation, synapto-dendritic elF4B is dephosphorylated at serine 406 in a rapid process that is mediated by protein phosphatase 2A. Such dephosphorylation causes a significant decrease in the binding affinity between elF4B and BC RNA translational repressors, enabling the factor to engage the 40S small ribosomal subunit for translation initiation. BC RNA translational control, mediated via elF4B phosphorylation status, couples neuronal activity to translational output, and thus provides a mechanistic basis for long-term plastic changes in nerve cells.
- 出版日期2014-10-27
