Adipose-Derived Mesenchymal Stromal Cells Seeded on Demineralized Bone Matrix Persist and Differentiate When Implanted in a Critical Sized Rat Femur Defect

作者：Ehrhart Nicole P; Chubb Laura; Shi Yaling; Flaumenhaft Elissa; Rose Ruth

来源：Journal of Biomaterials and Tissue Engineering, 2017, 7(4): 275-282.

DOI：10.1166/jbt.2017.1570

• Mesenchymal Stromal Cells
• Bone Tissue Engineering
• Demineralized Bone Matrix

Mesenchymal stromal cells (MSCs) have remarkable reparative capacities in a number of challenging disease states including repair of large bone defects. Survival of transplanted MSCs is increased when attached to a scaffold prior to surgical implantation. Here, the survival and fate of transplanted MSCs seeded on a demineralized bone matrix (DBM) carrier were evaluated following implantation in a critical-sized bone defect in an athymic recipient for 84 days. Forty-two athymic rats had a 5 mm defect created in the left femur following placement of a stabilization plate. Adipose-derived MSCs were isolated from green fluorescent protein (GFP) transgenic rats, seeded onto a DBM matrix and implanted into the defect. Bone formation was monitored by radiography. Immunohistochemistry and fluorescent microscopy was used to identifiy GFP-expressing cells within the repair tissue at timepoints up to 84 days. Robust bone formation occurred within the defect in all subjects. GFP-expressing cells were detectable within areas of new bone formation for up to 84 days. Co-localization of GFP and osteocalcin using immunohistochemistry indicated differentiation into osteoblastic lineages in transplanted cells. Transplanted MSCs seeded on DBM both persisted and differentiate into repair tissues within a bony defect for up to 84 days.

• 出版日期2017-4