A highly selective and ultrasensitive ratiometric fluorescent probe for peroxynitrite and its two-photon bioimaging applications

作者：Wang, Zuokai; Wu, Liu; Wang, Yawei; Zhang, Meng; Zhao, Ziyang; Liu, Caiyun^*; Duan, Qingxia; Jia, Pan; Zhu, Baocun^*

来源：Analytica Chimica Acta, 2019, 1049: 219-225.

DOI：10.1016/j.aca.2018.05.064

• Fluorescent probe
• Peroxynitrite (ONOO-)
• Ratiometric detection
• Hydroxynaphthalimide
• Two-photon bioimaging

In this work, taking full advantage of the intramolecular charge transfer (ICT) mechanism, a hydroxynaphthalimide-based ratiometric two-photon fluorescent probe RTP-PN was synthesized to detect ONOO-. Probe RTP-PN could accurately detect ONOO- in the range of 1.4 nM-1.4 mM with the detection limit of 1.4 nM by a ratiometric fluorescence spectroscopy method. Additionally, probe RTP-PN exhibited an ultrafast response for ONOO- than other various species including H2O2 and ClO-. Finally, probe RTP-PN was successfully adopted to detect intracellular ONOO- by the two-photon excitation microscopy.

• 出版日期2019-2-21
• 单位济南大学