Bfil, a restriction endonuclease from Bacillus firmus S8120, which recognizes the novel non-palindromic sequence 5 '-ACTGGG(N)(5/4)-3 '

作者：Vitkute J; Maneliene Z; Petrusyte M; Janulaitis A^*

来源：Nucleic Acids Research, 1998, 26(14): 3348-3349.

DOI：10.1093/nar/26.14.3348

A new type IIS restriction endonuclease Bfil has been partially purified from Bacillus firmus S8120. Bfil recognizes the non-palindromic hexanucleotide sequence 5'-ACTGGG(N)(5/4)-3' and makes a staggered cut at the fifth base pair downstream of the recognition sequence on the upper strand, producing a single base 3' protruding end.

• 出版日期1998-7-15