Age-related expression, enzymatic solubility and modification with advanced glycation end-products of fibrillar collagens in mouse lung

作者：Rolewska Paulina; Al Robaiy Samiya; Santos Alexander Navarrete; Simm Andreas; Silber Rolf Edgar; Bartling Babett^*

来源：Experimental Gerontology, 2013, 48(1): 29-37.

DOI：10.1016/j.exger.2012.04.012

• Collagen
• Aging
• Glycation
• Lung
• Mouse

Changes in the expression of fibrillar collagens and post-translational modifications with advanced glycation end-products (AGEs) are often associated with tissue aging. Less is known about age-related changes in mouse lung tissue. Therefore, we studied the expression level and AGE load of fibrillar collagens in lungs from young (<= 6 months), adult (15 months) and old (>= 25 months) mice. The mRNA expression level was reduced in adult and old mice compared with the young. Old mice also showed a reduced protein level, whereas the adults even had more collagen protein. Fractionating of the fibrillar collagens into enzyme-soluble and insoluble collagens revealed a reduced solubility of collagens in old age. The enzymatic solubility of fibrillar collagens correlated inversely with the AGE load in the insoluble collagen as detected by the AGE-related fluorescence. While the intensity of the AGE-related fluorescence was increased in fibrillar collagens in response to age, the fluorescing AGE variant argpyrimidine was less affected. In summary, aging causes a reduced expression, lower enzymatic solubility and increased AGE load of fibrillar collagens in mouse lung tissue, but not all changes occur gradually with age.

• 出版日期2013-1