Association of a synthetic bone graft and bone marrow cells as a composite biomaterial

作者：Rabiee S M^*; Mortazavi S M J; Moztarzadeh F; Sharifi D; Fakhrejahani F; Khafaf A; Ahmadi S A Houshiar; Nosoudi N; Ravarian R

来源：Biotechnology and Bioprocess Engineering, 2009, 14(1): 1-5.

DOI：10.1007/s12257-008-0089-1

• synthetic bone graft
• porous tricalcium phosphate
• bone marrow
• mechanical properties

Porous calcium phosphates have osteoconductive properties. The aim of this study was to obtain synthetic calcium phosphate bone graft substitute. X-ray diffraction was employed to investigate the formation of the beta-tricalcium phosphate (beta-TCP) phase. We evaluated the effects of bone marrow on the osteoconductivity and mechanical properties of synthetic bone graft (SG). SG cylinders loaded with bone marrow (SGBM) and SG alone were implanted into rabbits femoral condyle bone defects. Histological examinations revealed the resorption of the SG, trabecular bone with osteoblasts and osteoid substance around the implants, and colonization inside the porous beta-TCP by newly formed bone. Histomorphometry conducted after three months revealed the osteoid surface to be higher in SGBM than SG (p < 0.05). The compressive strengths of SG and SGBM were significantly higher than the anatomic control at all time periods. The elastic modulus of SBG and SGBM became weaker after implantation. The present results indicate that gB-TCP is a good matrix for bone marrow, which contributes osteoinductive properties in an orthotopic. The composite biomaterial may be useful in reconstructive bone surgery.

• 出版日期2009-2