Analysis of Steroid Hormones in Water Using Palmitate-Coated Magnetite Nanoparticles Solid-Phase Extraction and Gas Chromatography-Tandem Mass Spectrometry

作者：Ana Perez Rosa; Albero Beatriz; Luis Tadeo Jose; Molero Encarnacion; Sanchez Brunete Consuelo^*

来源：Chromatographia, 2014, 77(11-12): 837-843.

DOI：10.1007/s10337-014-2688-7

• Magnetic solid-phase extraction
• Magnetic nanoparticles
• Gas chromatography-tandem mass spectrometry
• Steroid hormones

A new extractive method based on magnetic solid-phase extraction using palmitate-coated magnetite nanoparticles coupled with gas chromatography-tandem mass spectrometry was developed for the extraction of estrone, androstenedione and progesterone hormones from water. The amount of nanoparticles and extraction time were optimized applying an experimental design to estimate simultaneously their influence on the recoveries. Results showed that the extraction efficiency was highly affected by the addition of salt and acetonitrile was the solvent used to elute the analytes from the nanoparticles. Derivatization via enolization of ketone group was carried out in the extract for the simultaneous derivatization of hydroxyl and ketone groups of steroid hormones previously to their chromatographic analysis. The extraction efficiencies of the nanoparticles were studied spiking at four concentration levels and recoveries from 94.6 to 109.7 % were obtained. The limits of detection ranged from 4 to 8 ng L-1. The developed method provided a preconcentration factor of 100. The present work shows that palmitate-coated magnetite nanoparticles have good applicability for the extraction of natural estrogenic hormones that could be pollutants in real water samples.

• 出版日期2014-6