Development of an Enzyme Mimic Using Self-Selection

作者：Gasparini Giulio; Dal Molin Marta; Corra Stefano; Galzerano Patrizia; Serimin Paolo; Prins Leonard J^*

来源：Israel Journal of Chemistry, 2013, 53(1-2): 122-126.

DOI：10.1002/ijch.201200080

• dynamic combinatorial chemistry
• enzyme catalysis
• supramolecular chemistry
• thermodynamic control
• transition states

The development of a serine protease model using a self-selection protocol is described. The developed system mimics the regeneration step of an enzyme involved in covalent enzyme catalysis. A transition-state analogue of a transesterification reaction is used to self-select functional groups able to accelerate ester cleavage. It is shown that the insertion of a tertiary amine substituent flanking the reaction center reinforces transition-state stabilization by directing the reactive center towards the self-selected functionality. In addition, the tertiary amine activates a bland (solvent) nucleophile for attack on an ester bond similar to what occurs in a serine protease. A quantitative correspondence is observed between the amplification factors and catalytic activity, illustrating the potential of the dynamic covalent capture strategy to precisely detect and quantify weak noncovalent interactions.

• 出版日期2013-2