A Spectrophotometric Assay for Robust Viability Testing of Seed Batches Using 2,3,5-Triphenyl Tetrazolium Chloride: Using Hordeum vulgare L. as a Model

作者：Del Egido Laura Lopez^*; Navarro Miro David; Martinez Heredia Victor; Toorop Peter E; Iannetta Pietro P M

来源：Frontiers in Plant Science, 2017, 8: e747.

DOI：10.3389/fpls.2017.00747

• formazan
• Hordeum vulgare
• seed-testing
• seed viability
• tetrazolium
• TTC

A comparative analysis was carried out of published methods to assess seed viability using 2,3,5-triphenyltetrazolium chloride (TTC) based assays of seed batches. The tests were carried out on seeds of barley (Hordeum vulgare cv. Optic) as a model. We established that 10% [w/v] trichloroacetic acid (TCA)/methanol is superior to the acetone and methanol-only based methods: allowing the highest recovery of formazan and the lowest background optical density (OD) readings, across seed lots comprising different ratios of viable and dead seeds. Themethod allowed a linear-model to accurately capture the statistically significant relationship between the quantity of formazan that could be extracted using the method we developed and the seed temperature-response, and seed viability as a function of artificially aged seed lots. Other quality control steps are defined to help ensure the assay is robust and these are reported in a Standard Operating Procedure.

• 出版日期2017-5-16