A Tandem Enzymatic sp(2)-C-Methylation Process: Coupling in Situ S-Adenosyl-l-Methionine Formation with Methyl Transfer

作者：Sadler Joanna C; Humphreys Luke D^*; Snajdrova Radka; Burley Glenn A^*

来源：ChemBioChem, 2017, 18(11): 992-995.

DOI：10.1002/cbic.201700115

• alkylation
• biocatalysis
• methyltransferase
• multi-enzyme reaction
• S-adenosylmethionine

A one-pot, two-step biocatalytic platform for the regiospecfic C-methylation and C-ethylation of aromatic substrates is described. The tandem process utilises SalL (Salinospora tropica) for in situ synthesis of S-adenosyl-l-methionine (SAM), followed by alkylation of aromatic substrates by the C-methyltransferase NovO (Streptomyces spheroides). The application of this methodology is demonstrated for the regiospecific labelling of aromatic substrates by the transfer of methyl, ethyl and isotopically labelled (CH3,)-C-13 (CD3)-C-13 and CD3 groups from their corresponding SAM analogues formed in situ.

• 出版日期2017-6-1