Embryo toxicity of pesticides and heavy metals to the ramshorn snail, Marisa cornuarietis (Prosobranchia)

作者：Sawasdee Banthita^*; Koehler Heinz R

来源：Chemosphere, 2009, 75(11): 1539-1547.

DOI：10.1016/j.chemosphere.2009.01.085

• Atrazine
• Embryo toxicity test
• Imidacloprid
• Marisa cornuarietis
• Nickel
• Zinc

An invertebrate embryo toxicity test with the ampullariid snail, Marisa cornuarietis, to assess the toxicity of pesticides and heavy metals recently was established. Snail embryos were treated with atrazine (100, 1000, 10000, and 30000 mu g/L), imidacloprid (10000, 25 000, and 50000 mu g/L). Ni(2+) (0.1, 1, 10, and 100 mu g/L) or Zn(2+) (100, 200, 500, 1000, 2000, and 5000 mu g/L). The effect of these substances was examined by monitoring the following endpoints: mortality, formation of tentacles and eyes, heart rate, hatching, and weight after hatching. Effects in term of a significant delay on the formation of both tentacles and eyes were found after treatment with 100 mu g/L Ni(2+) or 200 mu g/L Zn(2+). The heart rate was shown to significantly decrease at 25 000 mu g/L imidacloprid or 1000 mu g/L Zn(2+). At 100 mu g/L atrazine, 10 mu g/L Ni(2+) or 1000 mu g/L Zn(2+) a significant delay in hatching became visible. No significant mortality was observed for the tested concentrations of atrazine, imidacloprid, or Ni(2+), while 5000 mu g/L Zn(2+) resulted in 100% mortality after 10 d. The weight of freshly hatched individuals remained unaffected in all treatments. On the basis of the lowest observed effect concentrations (LOECs) recorded, we could show the M. cornuarietis embryo toxicity test (MariETT) to react up to three orders of magnitude more sensitive (for metals) and at least one order of magnitude more sensitive (for the tested organics) than the established Danio, rerio embryo test.

• 出版日期2009-6