Methods to detect antifibrillarin antibodies in patients with systemic sclerosis (SSc): A comparison

作者：Garcia JH; Osuna MD; Castrejon FM; Enriquez LG; Reyes PA; Hermosillo JJC^*

来源：Journal of Clinical Laboratory Analysis, 2004, 18(1): 19-26.

DOI：10.1002/jcla.20003

• immunofluorescence
• Western blot
• immunoprecipitation snoRNAs
• rheumatic diseases

Autoantibodies against nucleolar antigens are common in systemic sclerosis (SSc). They include autoantibodies against fibrillarin (Fb), which are serological markers for SSc. Fb is associated with the evolutionally-conserved box C/D of small nucleolar RNAs (snoRNAs). We compared indirect immunofluorescence (IIF), Western blot (WB), and immunoprecipitation (IPP) of total small RNAs assays to determine which of these techniques is most specific for the detection of snoRNPs. We also examined the frequency and specificity of autoantibodies from SSc patients to snoRNAs, snRNAs, and scRNAs, and concluded that 1) IIF can not determine autoantibody specificity against Fb, 2) 36% of SSc sera were false-negative by WB, and 3) by IPP, anti-Fb autoantibodies from SSc patients can bind U3, U8, U13, U15, and U22 snoRNAs.

• 出版日期2004