MicroRNA-552 promotes hepatocellular carcinoma progression by downregulating WIF1

作者：Li, Chao; Wang, Zi; Chen, Shuangjiang; Zhang, Jingyao; Qu, Kai; Liu, Chang^*

来源：International Journal of Molecular Medicine, 2018, 42(6): 3309-3317.

DOI：10.3892/ijmm.2018.3882

• microRNA-552
• hepatocellular carcinoma
• invasion
• epithelial-mesenchymal transition
• Wnt inhibitory factor 1

MicroRNAs (miRNAs/miRs) are involved in the metastasis of hepatocellular carcinoma (HCC). In the present study, it was demonstrated that miR-552 was upregulated in HCC tissues. High miR-552 expression was associated with malignant clinicopathological features and decreased survival rates. The in vitro results indicated that miR-552 overexpression promoted migration, invasion and epithelial-mesenchymal transition in Hep3B cells. However, the knockdown of miR-552 inhibited its oncogenic roles in Huh-7 cells. Additionally, Wnt inhibitory factor 1 (WIF1) was demonstrated to be a direct target of miR-552 in Hep3B and Huh-7 cells. Additional experiments identified that miR-552 promotes beta-catenin expression by increasing the phosphorylation of GSK3 beta at Ser9. In conclusion, the results suggested that miR-552 may promote HCC progression by blocking WIF1-mediated GSK3 beta dephosphorylation. miR-552 may be a biomarker for predicting the outcomes of patients with HCC.

• 出版日期2018-12
• 单位西安交通大学