Adipocyte fatty acid-binding protein: An important gene related to lipid metabolism in chicken adipocytes

作者：Shi, Hui; Wang, Qigui; Wang, Yuxiang; Leng, Li; Zhang, Qingqiu; Shang, Zhouchun; Li, Hui^*

来源：Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology, 2010, 157(4): 357-363.

DOI：10.1016/j.cbpb.2010.08.005

• A-FABP
• Lipid accumulation
• Lipolysis
• Chicken

Fatty acid-binding proteins (FABPs) may facilitate the diffusion of fatty acids within the cytoplasm. Adipocyte FABP (A-FABP) null mice exhibit altered rates of lipolysis, and reprogrammed expression of genes related to lipid metabolism. In the current study, chicken adipocytes with short hairpin RNA-mediated knockdown and cDNA overexpression of A-FABP were used to investigate the role of chicken A-FABP in lipid metabolism of adipocytes. The results showed that there was no significant change of lipid metabolism in the A-FABP interference adipocytes. However, in the A-FABP overexpression adipocytes, the lipolysis was increased significantly at 36 h (p<0.05), and lipid accumulation was increased significantly at 48, 60 and 72 h (p<0.05). The expression of PPAR gamma and perilipin genes were upregulated, while the expression of E-FABP gene was downregulated after transfection with pcDNA3.1/A-FABP (p<0.05). The present study suggested that A-FABP gene might affect the lipid metabolism through the PPAR gamma pathway, and the function between A-FABP and E-FABP gene was compensatory in the chickens.

• 出版日期2010-12
• 单位东北农业大学