Hemoglobin assay in anemic patients with a photothermal spectral-domain optical coherence reflectometric sensor

作者：Kim Hun; Song Sungwook; Yim Jinyeong; Kim Hyun Ok; Joo Chulmin^*

来源：Clinica Chimica Acta, 2015, 439: 71-76.

DOI：10.1016/j.cca.2014.10.001

• Anemia
• Hemoglobin concentration
• Optical sensor
• Optical interferometer
• Photothermal effect

Background: We recently introduced an optical sensor, termed photothermal spectral-domain optical coherence reflectometer (PT SD-OCR), capable of direct measurement of hemoglobin concentration ( [Hb]) without chemicals and pre-processing of blood. PT SD-OCR measures [Hb] by quantifying refractive index changes of blood samples due to photothermal effect under the irradiation of 532-nm light. We evaluated diagnostic accuracy and precision of PT SD-OCR in anemic patients.
Methods: We used PT SD-OCR to measure [Hb] in 50 anemic patients, and examined its accuracy against a hematology analyzer (ADVIA (R) 2120i, Siemens AG). Its precision was assessed by examining the SD and CV based on 20 repeated measurements on 3 blood samples. Its performance was also compared with a hemoglobinometer (HemoCue (R) 201 +, HemoCue (R)).
Results: The PT SD-OCR demonstrated good correspondence with the hematology analyzer with a Pearson's correlation coefficient of 0.992 and a bias of -0.055 g/dl. Standard deviation and CV were measured to be <0.25 g/dl and <2.05%.
Conclusions: Simple and chemical-free operation of PT SD-OCR enabled rapid and accurate measurement of [Hb] in anemic patients. The sensor is expected to facilitate clinical procedures related to blood-related disorders in patient care. 2014 Elsevier B.V.

• 出版日期2015-1-15